Zeiss 510 Meta Laser Scanning Confocal Microscope (LSCM)

The Zeiss 510 Meta LSCM is equipped with 2 confocal fluorescence detectors, a confocal polychromatic detector and a transmitted light detector. The system has 3 lasers which are a 40 mW argon,1 mW 543 green HeNe, and 5 mW 633 nm HeNe laser. The system is based on a Zeiss AxioImager Z1 microscope and has both standard epifloresence and DIC capabilities. The lenses available on the microscope are as follows: 10x Plan Neofluor, 20x Plan Apo, 40x Plan Neoflour oil immersion, 63x Plan Apo oil and water dippable C-apochromat 40x lens.

JEOL 1200EX Transmission Electron Microscope

This Electron Microscope is equipped with a Gatan Bioscan digital camera.

Nikon PCM 2000 laser scanning confocal microscope

The Nikon PCM 2000 LSCM is equipped wth 2 confocal florescence detectors and a transmitted brightfield detector. The system has 3 lasers: a 40mW argon, .1mW 543 Green HeNe, and a 10mW 633 HeNe laser. The lenses available for use include a Plan Apo 4x, Plan Apo10x, Plan Apo 20x, Plan Apo 40x oil, Fluor dippable 40x water, Plan Apo 60x oil, Fluor dippable 60x water and Plan Apo 100x oil.

Live cell imaging system for time-lapse imaging

This system is based on an Olympus BX50WI fixed stage microscope equipped with fluorescence and DIC optics. The lenses are waterimmersion lenses (10x, 20x, 40x, 60x). The stage has an ASI computer controlled x,y stage as well as a computer-controlled Ludl z-step motor. A Harvard apparatus temperature controllerfor maintaining 0.1 degree temperature control. The camera is a Photometrics Quantix cooled CCD camera with Uniblitz shutters on both the fluorescence and DIC light sources. The system control and analysis software is Compix, a Hamamatsu-owned company.

Dynamic Intensity Analysis system for calcium imaging

This system is based on an Olympus BX50WI fixed stage microscope equipped with epifluorescence and DIC optics. The lenses are waterimmersion lenses (10x, 20x, 40x, 60x). The camera is a Hamamatsu Orca 285 camera with a Uniblitz shutter on DIC transmittal light sources. The fluorescence light source has a Ludl high speed shutter and filter wheel. The focus in z is controlled by a Ludl z-step motor. The system control and analysis software is Compix, a Hammamatsu -owned company.